CH | CHF
-
-
-
-
- Services pour bioprocédés
- Services pour centrifugeuse et rotors
- Service pour pipette
- Services pour Mastercycler
- Services pour automates de pipetage
- Services pour congélateurs
- Services pour incubateurs
- Services pour agitateurs
- Services pour appareils de photométrie
- Service de contrôle de la température et de l’agitation
-
-
-
-
-
- Services pour bioprocédés
- Services pour centrifugeuse et rotors
- Service pour pipette
- Services pour Mastercycler
- Services pour automates de pipetage
- Services pour congélateurs
- Services pour incubateurs
- Services pour agitateurs
- Services pour appareils de photométrie
- Service de contrôle de la température et de l’agitation
-
CH | CHF
-
- Benchtop Centrifuges
- Floor-Standing Centrifuges
- Refrigerated Centrifuges
- Microcentrifugeuses
- Centrifugeuses multi-fonctions
- Centrifugeuses haute vitesse
- Ultracentrifugeuses
- Concentrateur
- High-Speed and Ultracentrifugation Consumables
- Accessoires
- Tubes
- Plaques
- Gestion des appareils
- Gestion des échantillons et des informations
- Produits IVD
96-Wells at Once – Faster and More Reproducible Cell Assays
Lab Academy
Cell Based Assays are an established standard tool in life science and performed in 96 and 384-well plates. They require multichannel pipetting to ensure quick, efficient, and reproducible handling – especially when multiple plates need to be processed. A manual 12-channel pipette, an electronic 12-channel pipette and a semi-automated 96-channel electronic pipette (epMotion 96) were compared for cell seeding, cytotoxic agent and assay reagent addition.
Cell viability was evaluated after treatment with a staurosporine dose-response curve. Profiles generated were comparable for each instrument used for cell seeding and reagent adding. Nevertheless, with a manual pipetting system, the assay reproducibility was affected as indicated by higher standard deviations.
Lire moins
With all assay performance parameters studied (signal-to-background ratio, Z’-factor, intra-plate and inter-plate CV), it was demonstrated that the assay robustness and reproducibility were significantly better when an electronic pipette (epMotion 96 or electronic pipette) was used.
Lire moins
Among all instruments tested, the epMotion 96 appears as the fastest option since a complete cell-based assay requires only 4 minutes, that means 5 times less hands-on time than what is needed with the manual pipette.
- to read the whole Application Note.
- to read the whole Application Note.
Lire moins